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. 2020 Jul 7;11:3394. doi: 10.1038/s41467-020-17110-x

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a, b Representative normalised Per2::Luciferase traces for (a) VIP^Cre SCN and (b) VPAC2^Cre SCN conditionally expressing either TdTomato (Black) or the diphtheria toxin receptor (DtR) (VIP^Cre: blue, VPAC2^Cre: pink). The traces show ongoing ensemble oscillation before, during and after wash-out of diphtheria toxin (Dtx) addition, and the insets to the right show expanded views of the oscillations post-washout. c, d Bioluminescent micrographs of a single cycle of bioluminescence for (c) VIP^DtrR and (d) VPAC2^DtR SCN before addition of Dtx (upper) and post-washout (lower). Scale bars = 100 µm. e, f Raster plots showing cellular bioluminescence intensity through time for (e) VIP^DtR and (f) VPAC2^DtR SCN recordings conditionally expressing DtR before, during and after addition of Dtx. Alongside are representative Rayleigh plots of the corresponding slices showing phase-coherence before addition of Dtx (black) and post-washout (VIP^Cre: blue; VPAC2^Cre: pink). Individual points represent individual oscillator circular phases; arrows represent mean circular phase for the slice and condition. g Summary Rayleigh vector lengths before Dtx treatment (hollow bars) and post-washout (solid bars) for VIP^Cre (blue, n = 6) and VPAC2^Cre (pink, n = 5) SCN conditionally expressing DtR (**p < 0.01). Individual points represent individual slice values; bars represent mean ± SEM. Statistics: g repeated-measures two-way ANOVA with Sidak’s correction for multiple comparisons. Only significant comparisons (p < 0.05) are shown. Source data are provided as a source data file.