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. 2020 Jul 7;11:3394. doi: 10.1038/s41467-020-17110-x

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 7 — a Representative Per2::Luciferase PMT traces of Cry-null SCN, before and during initiation of conditional Cry1-expression, and after AAV washout (WO) in VIP^Cre (blue, left), VPAC2^Cre (pink, middle) and VIP^Cre;VPAC2^Cre slices (purple, right). Arrows indicate AAV addition and washout. b Summary periods for pan-neuronal (Syn, grey, n = 5) or VIP^Cre;VPAC2^Cre (purple, n = 12) Cry1 expression. c Paired goodness of fit (GOF) measures for baseline (black) and post-washout (coloured) for VIP^Cre (blue, n = 18), VPAC2^Cre (pink, n = 10) and VIP^Cre;VPAC2^Cre (purple, n = 17) SCN (p < 0.0001). d Percentage of Cry1::EGFP positive SCN cells versus the GOF for VIP^Cre (blue, n = 8), VPAC2^Cre (pink, n = 3) and VIP^Cre;VPAC2^Cre (purple, n = 13) conditional Cry1-expressing SCN. e Representative CoL trajectories showing relative spatial organisation of VIP^Cre (blue, n = 9), VPAC2^Cre (pink, n = 9) and VIP^Cre;VPAC2^Cre (purple, n = 9) in Cry-null SCN conditionally expressing Cry1 (left) alongside summary trajectories (right) including wild type (grey, n = 9) (*p = 0.047, ***p < 0.001). Lighter lines indicate individual cycles, darker lines indicate the mean of the individual cycles. f Summary Rayleigh vector length for wild-type SCN (grey, n = 9), or VIP^Cre (blue, n = 9), VPAC2^Cre (pink, n = 9) and VIP^Cre;VPAC2^Cre (purple, n = 9) Cry-null SCN conditionally expressing Cry1 (*p = 0.027, **0.004, ***p = 0.0002). g Representative categorical, annotated phase map showing spatial phase distribution in VIP^Cre;VPAC2^Cre Cry1-initiated SCN, asterisk indicates third ventricle. In all plots: Individual points (paired/unpaired) represent individual slices; bars represent mean ± SEM. Statistics: b unpaired two-tailed t-test, c repeated-measures two-way ANOVA; e, f Ordinary one-way ANOVA. Only significant comparisons (p < 0.05) are shown. Source data are provided as a source data file.