Fig. 3. Nampt-depletion increases spindle length but not anaphase-spindle elongation.

a Shown are panels comprised of selected frames from representative time-lapse series of oocytes with SiR-tubulin-labelled spindles and expressing H2B-RFP to label chromosomes. Scale bar, 10 μm. Representative images from three independent experiments are shown. b Spindle length at the time when bipolar spindles first formed. c Measurements of spindle lengths at 5 min prior to anaphase-onset (pre-anaphase), anaphase-onset and 10 min post-anaphase-onset. d Percentage increase in spindle length during anaphase relative to pre-anaphase-spindle length. e Spindle width at the time when bipolar spindles first formed. f Spindle width at pre-anaphase, anaphase-onset and 10 min post-anaphase-onset. Oocyte numbers are shown in parenthesis from three independent experiments (b–e, f). Data in graphs are presented as the mean ± SEM. P-values are shown in the graphs, ns denoted P > 0.05. Statistical comparisons were made using either two-tailed Student’s t-test in b and e or two-way repeated measures ANOVA in c, d and f. Source data are provided as a Source Data file.