Fig. 4. Post-anaphase-onset spindle acceleration.

a, b Schematics depicting the spindle displacement measurements plotted in c and d. c, d Posterior pole displacement (y − y⁰ (a)) (c) and midzone displacement (z − z⁰; (b)) (d) during anaphase. e Mean spindle migration speeds relative to anaphase-onset for mock-depleted and Nampt-depleted oocytes. Note the increase in speed in mock-depleted oocytes that is absent after Nampt-depletion. f, g Shown are panels comprise selected brightfield and fluorescence frames from representative time-lapse series of mock-depleted (f) and Nampt-depleted (g) oocytes. Time, h:min relative to anaphase-onset. Scale bars, 10 µm. Representative images from three independent experiments are shown. h Tracks of spindle migration speeds during anaphase for the oocytes shown in f and g. White marks correspond to the position of the spindle at each consecutive time point shown in f and g. Note that in the mock-depleted oocyte shown, spindle speed is maximal (red track) between anaphase-onset and 10-min post-anaphase-onset. Note that the distance between white tick marks does not correspond to the distance moved by spindles as this track is a 2D representation of motion that occurred in three dimensions. Oocyte numbers are shown in parenthesis from three independent experiments (c–e). Data in graphs are presented as the mean ± SEM. P-values are shown in the graphs, ns denoted P > 0.05. Statistical comparisons were made using two-way repeated measures ANOVA in c and d. See also Supplementary Movies 4 and 5. Source data are provided as a Source Data file.