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. 2020 Jul 7;11:3393. doi: 10.1038/s41467-020-17088-6

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 7 — a, b Spindle lengths at the time when bipolar spindles first formed (a) and 10 min post-anaphase-onset (b). c Ratio of PB width to oocyte diameter. d Proportion of oocytes with large PBs. See Methods for further details. e Mean spindle migration speeds relative to anaphase-onset for mock-depleted and Mos-depleted oocytes. f Proportion of oocytes with a single furrowing site. g ControlMO- and MosMO-injected oocytes were immunoblotted for Nampt. Vinculin served as a loading control (left); n = 15 oocytes per lane. Experiments were repeated three times. Quantification of Nampt protein levels (right). Note that Nampt levels were markedly reduced following Mos-depletion. Oocyte numbers are shown in parenthesis from three independent experiments (a–f). Data in graphs are presented as the mean ± SEM. Data points are shown in d, f and g. P-values are shown in the graphs. Statistical comparisons were made using either two-tailed Student’s t-test in a, f and g or one-way ANOVA in b–d. Source data are provided as a Source Data file.