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. 2020 Jul 7;10:11143. doi: 10.1038/s41598-020-68113-z

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Creation of Gprc6a-^KGKY-knockin mice. (a) Structure of Gprc6a exon 6, knockin sequence and genotype primers and Dra I restricted enzyme location. The orange box shows the KY location in blue arrow. The yellow arrow shows the ssOBD-HDR location. The green arrow shows sgRNA location. (b) Comparison of the body weight in wild type and Gprc6a-^KGKY-knockin mice at age from 8 to 54 weeks. (c) Site specific brown and white fat content in 20 week-old male mice. Interscapular brown fat (iBAT) and white inguinal fat (iFAT) and epididymal fat (eFAT). Values represent the mean ± SEM. *Significant difference between wild type mouse Gprc6a-^RKLP and Gprc6a-^KGKY-knockin mice (P < 0.05, Student’s t test; n = 6).