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. 2020 Jul 7;10:11186. doi: 10.1038/s41598-020-68019-w

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Dermal capillary vessels lose pericyte coverage upon local PGE2 injection. Mice were injected with PGE2 (200 ng) or vehicle (DMSO) into dorsal skin on Day 0 and Day 1, then skin samples were collected and tissue sections were immunostained for NG2 (green), CD31 (red) and DAPI (blue). A representative micrograph of microvessels is shown for each condition. The pictures were taken using a 60 × objective lens. The depth of confocal scanning was 4.35 μm; therefore, winding microvessels are seen in fragments in these pictures. The right picture panel shows NG2⁺ pericytes that are dissociated from the CD31⁺ endothelium in the PGE2-treated skin. Blood vessel coverage by NG2⁺ pericytes is presented in a graph with an arbitrary unit. The analysis was conducted in total of > 68 micrographs of different areas in 5 skin samples for each condition. Each dot represents one micrograph. Student t-test, ****p < 0.0001.