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. 2020 Jul 7;10:11186. doi: 10.1038/s41598-020-68019-w

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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PGE2 impairs N-cadherin and Cx43-mediated pericyte–EC interaction. HBVPs (green) were treated with PGE2 for 72 h prior to coculture with HUVECs (red) on tissue culture plates. Cells were stained for N-cadherin (top panels) and Cx43 (bottom panels) 18 h later. Immunofluorescence intensities of these junctional proteins are shown in surface plots with a color-coded scale created by Image J (right panel, red for the strongest and dark blue for the weakest fluorescence signals). At least 10 different areas of the coculture were analyzed for each condition. A representative area is shown for each. Scale bar 50 μm.