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. 2020 Jul 7;10:11186. doi: 10.1038/s41598-020-68019-w

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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PGE2 downregulates N-Cadherin and Cx43 expression at the protein level. (a) Time-course western blot analysis of N-cadherin and Cx43 expression in HBVPs treated with PGE2 for 12, 24, or 48 h. GAPDH was used as a loading control. (b, c) HBVPs were treated with PGE2 or control DMSO. N-cadherin (b) and Cx43 (c) expression was then analyzed by quantitative RT-PCR at 48 h post-treatment. The mRNA extracts were pooled from three different cell culture dishes, and the RT-PCR was performed in triplicates, and repeated in two independent experiments. The mRNA expression levels were normalized to cyclophilin A. Fold change is shown relative to the control. One-way ANOVA test, ns, not significant.