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. 2020 Jul 7;10:11162. doi: 10.1038/s41598-020-66630-5

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Overview of experimental Design. Primary mesenchymal cell populations were isolated from control (N = 3) and IPF (N = 3) human lung tissue and used between passages 2 and 6. FACS was used to isolate a single cell suspension of SSEA4^hi cells (hereafter referred to as MPCs). Cells were subjected to live/dead assessment, microscopic evaluation to ensure single cells in each chamber and sequenced on the Fluidigm C1 platform according to the manufacturer’s protocol.