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. 2020 Jul 7;10:11162. doi: 10.1038/s41598-020-66630-5

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Cells expressing CD44, SSEA-4 and MARCKS reside in the cellular perimeter region of the fibroblastic focus. An Idiopathic Pulmonary Fibrosis (IPF) specimen was serially sectioned at 4 μm and processed for histology, immunohistochemistry (IHC) and immunofluorescence (IF). IHC: Representative images for Hematoxylin and Eosin (H&E) staining (scale bar represents 50 μm left and 20 μm right) with an asterisk labeling a fibroblastic focus; Immunostaining for anti-procollagen type I (brown, scale bar 20 μm); anti-CD44 (red, scale bar 20 μm, dashed outline box, scale bar 20 μm); anti-SSEA4 (brown, scale bar 20 μm, dashed outline box, scale bar 10 μm). Lower panel: Immunostaining anti-SSEA-4 (green), MARCKS (red), DAPI (blue, scale bar 20 μm). A small apoptotic body is noted adjacent to the cell on the right. Immunofluorescence images obtained at the perimeter of the fibroblastic focus.