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. Author manuscript; available in PMC: 2021 Jan 1.
Published in final edited form as: Nitric Oxide. 2019 Oct 8;94:1–8. doi: 10.1016/j.niox.2019.10.005

Figure 2. Concentration-dependent nitrate uptake and metabolism in myotubes.

Figure 2.

Primary human skeletal muscle cells (5 × 105 cells) were plated in a 60-mm dish and differentiated into myotubes in differentiation medium for 5 days. Myotubes were incubated with 10, 100, 500 and 1000 μM nitrate at 37°C for 40 minutes. After washing with PBS, cell lysates were collected in lysis buffer. Intracellular nitrate (A) and nitrite (B) levels were measured by chemiluminescence and normalized to protein concentration. Data are mean ± SEM (n=5). *P < 0.05 compared with control and tested by repeated measured ANOVA with Tukey’s multiple comparison.