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. Author manuscript; available in PMC: 2021 Jan 1.
Published in final edited form as: Nitric Oxide. 2019 Oct 8;94:1–8. doi: 10.1016/j.niox.2019.10.005

Figure 3. The expression of molybdenum-containing proteins and NOS1 during differentiation of myoblasts into myotubes.

Figure 3.

Primary human skeletal muscle cells (5 × 105 cells) were plated in a 60-mm dish. Cell lysate was collected before (undifferentiated myoblasts or UD) and 1 (D1), 3 (D3) and 5 (D5) days after differentiation. The expressions of xanthine oxidase (A), aldehyde oxidase (B), MARC-1 (C), MARC-2 (D) and NOS1 (E) were measured by western blots. Data are mean ± SEM (n = 4-6). *P < 0.05 compared with undifferentiated cells (UD) and tested by ANOVA with Tukey’s multiple comparison.