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. Author manuscript; available in PMC: 2021 Jan 1.
Published in final edited form as: Nitric Oxide. 2019 Oct 8;94:1–8. doi: 10.1016/j.niox.2019.10.005

Fig. 4. Increases of nitrite (A) and cGMP (B) levels in myotubes.

Fig. 4.

IBMX (500 μM) and/or ODQ (10 μM) were pre-incubated in myotubes for 15 min in CO2 incubator. Nitrite at a concentration of 1 mM was added to myotubes and incubated at 37 °C for 40 min. Cell lysates were collected in 0.1 M HCL containing 1% Triton X-100. Intracellular cGMP and nitrite levels were measured by ELISA kit and chemiluminescence. Data are mean ± SEM (n = 5). *P < 0.05 compared with other bar graphs and tested by ANOVA with Tukey’s multiple comparison.