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. Author manuscript; available in PMC: 2021 Jan 1.
Published in final edited form as: Nitric Oxide. 2019 Oct 8;94:1–8. doi: 10.1016/j.niox.2019.10.005

Figure 5. Anion-transporter mediated nitrate uptake in myotubes.

Figure 5.

(A) DIDS inhibition of nitrate uptake into myotubes. Myotubes were first incubated with DIDS for 30 min, washed with PBS and then incubated with nitrate. Intracellular nitrate levels were measured in myotubes lysate after 10 minutes incubation with nitrate (1 mM). The expression of CLC1 (B) and sialin (C) during the differentiation of myotubes were confirmed by Western blot. Data are mean ± SEM (n=3). *P < 0.05 compared with control and tested by unpaired t-test.