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. Author manuscript; available in PMC: 2021 Aug 5.
Published in final edited form as: Behav Brain Res. 2020 May 16;391:112625. doi: 10.1016/j.bbr.2020.112625

Figure 5. Shoaling behavior and validation with acute exposure to nicotine or ketamine.

Figure 5.

(A) Shoaling was assessed with a school of 4 fish in a tank containing 50 ml fish water. Endpoints included nearest neighbor distance (NND), inter-individual distance (IID), and swimming speed (cm/s). (B) Zebrafish at 28dpf were exposed to 24.6 μM nicotine for 30 s or 72.9 μM ketamine for 6 min as well as their corresponding vehicle controls (0 μM). Values plotted are mean ± SD (N = 10 tests with each test includes 4 fish). Asterisk indicates statistical significance at P < 0.05 (T-test).