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. 2020 Jul 1;8:553. doi: 10.3389/fcell.2020.00553

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Copyright © 2020 Sun, Yu, Li and Sun.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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ADNP forms complexes with BRG1 and CHD4. (A) A table showing representative ADNP interacting proteins by IP followed mass-spectrometry assay. (B) Schematic representation of wild type ADNP, BRG1 and CHD4, and their truncated mutant forms. (C) FLAG-tagged ADNP-N pulled down HA-tagged CHD4-C in 293T. (D) Endogenous BRG1 pulled down ADNP in ESCs. (E) FLAG-tagged ADNP-C pulled down HA-BRG1-N in 293T. (F) FLAG-tagged ADNP-C pulled down HA-BRG1-N using a TNT system. (G) FLAG-CHD4-C pulled down HA-BRG1-N in 293T cells. (H) HA-BRG1-N pulled down FLAG-CHD4-C in 293T. (I) FLAG-tagged pulled down HA-BRG1-N using a TNT system. (J) Sequential IP showing that ADNP, CHD4 and BRG1 could form a triplex in ESCs. (K) A cartoon showing the interaction of all three factors, based on the mapping results. All WB and IPs were repeated at least two times. Shown are representative images.