Supplemental Figure S10.
Aberrant Notch but not Yap activation in DDC-treated RictorLKO mice. A: Immunohistochemical staining of Yap on livers of Rictorfl/fl mice and RictorLKO mice with DDC diet. B: Western blot analysis of relative protein expression in liver tissues. Glyceraldehyde-3-phosphate dehydrogenase (Gapdh) was used as loading control. C: Quantitative real-time RT-PCR analysis of liver tissues from Rictorfl/fl and RictorLKO mice with DDC diet. At least three mice in each group were assayed. Data are expressed as means ± SEM (C). ∗P < 0.05, ∗∗P < 0.01. Scale bars = 100 μm (A). KO, knockout; p-, phosphorylated. Hes1, hes family bHLH transcription factor 1; Hes5, hes family bHLH transcription factor 5; HeyL, hairy/enhancer-of-split related with YRPW moti-like; KO, knockout; p-, phosphorylated; Taz, transcriptional coactivator with PDZ-binding motif.