Supplemental Figure S4.
Bile acid synthesis, gluconeogenesis, and lipogenesis pathways are not the major mTORC2 targets in this DDC-induced injury model. Quantitative real-time RT-PCR analysis of genes involved in gut Fgf15 (A), bile acid synthesis (B), gluconeogenesis (C), and lipogenesis (D) in DDC-treated Rictorfl/fl and RictorLKO mouse liver tissues. At least three mice in each group were assayed. Data are expressed as means ± SEM (A–D). ∗∗P < 0.01. Fasn, fatty acid synthase. Acaca, acetyl-CoA carboxylase alpha; Acly, ATP citrate lyase; Cyp7a1, cytochrome P450 family 7 subfamily A member 1; Fasn, fatty acid synthase; Fgf15, fibroblast growth factor 15; Fxr, farnesoid X receptor; G6pc, glucose-6-phosphatase catalytic subunit; Pc, Pyruvate carboxylase; Pck1, phosphoenolpyruvate carboxykinase 1; Srebf1, sterol regulatory element binding transcription factor 1.