Supplemental Figure S8.

mTORC1 signaling is dispensable in DDC-induced ductular reaction. A: Scheme of the experiment. B: Hematoxylin and eosin (H&E) and immunohistochemistry staining of cytokeratin 19 (CK19) on livers of rapamycin- and vehicle-treated mice with DDC diet. C: Quantification of CK19 staining. D: Western blot analysis of relative protein expression in liver tissues. Glyceraldehyde-3-phosphate dehydrogenase (Gapdh) was used as loading control. At least three mice in each group were assayed. Data are expressed as means ± SEM (C). Scale bars = 100 μm (B). 4Ebp1, 4E-binding protein 1; p-, phosphorylated; t-, total. 4Ebp1, eukaryotic translation initiation factor 4E-binding protein 1; mTOR, mammalian target of rapamycin; p-, phosphorylated; Rps6, ribosomal protein S6; t-, total.