Supplemental Figure S9.
Fatty acid synthase (Fasn) signaling is dispensable in DDC-induced ductular reaction. A: Scheme of the experiment. B: Hematoxylin and eosin (H&E) and cytokeratin 19 (CK19) immunohistochemistry staining of CK19 on livers of Fasnfl/fl mice and FasnLKO mice with DDC diet. C: Quantification of CK19 staining. D: Western blot analysis of relative protein expression in liver tissues. Glyceraldehyde-3-phosphate dehydrogenase (Gapdh) was used as loading control. At least three mice in each group were assayed. Data are expressed as means ± SEM (C). Scale bars = 100 μm (B). KO, knockout; Rps6, ribosomal protein S6; p-, phosphorylated; WT, wild type.