Figure 2.

Genetic Rictor ablation impairs ductular reaction. A: Scheme of the experiment. B: Gross image and hematoxylin and eosin (H&E) and immunohistochemical staining of cytokeratin 19 (CK19) and sex determining region Y-box 9 (Sox9) proteins in the livers of Rictor^fl/fl and Rictor^LKO mice fed 3,5-diethoxycarbonyl-1,4-dihydrocollidine (DDC) diet. C–E: Quantification of CK19 (C), Sox9⁺ ductular-like cell (D), and Sox9⁺ hepatocyte (E) staining. At least three mice per group were assayed. Data are expressed as means ± SEM (C–E). ∗∗P < 0.01, ∗∗∗∗P < 0.0001. Scale bars = 100 μm (B).