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. 2020 Jul;190(7):1414–1426. doi: 10.1016/j.ajpath.2020.03.010

Figure 7.

Figure 7

Rictor deficiency in hepatocytes has no effect on ductular reaction. A: Scheme of the experiment. B: Gross image and hematoxylin and eosin (H&E) and immunohistochemical staining of cytokeratin 19 (CK19) and sex-determining region Y-box 9 (Sox9) in the livers of AAV-Cre-Rictor and AAV-Null mice under 3,5-diethoxycarbonyl-1,4-dihydrocollidine (DDC) diet. Arrowheads indicate Sox9+ hepatocytes. C–E: Quantification of CK19+ cells (C), Sox9+ ductular-like cells (D), and Sox9+ hepatocytes (E). F: Western blot analysis of mTORC2/Akt signaling cascade in AAV-Cre-Rictor and AAV-Null mouse liver under DDC diet. Glyceraldehyde-3-phosphate dehydrogenase (Gapdh) was used as loading control. At least three mice per group were assayed. Data are expressed as means ± SEM (C–E). ∗∗∗∗P < 0.0001. Scale bars = 100 μm (B). 4Ebp1, eukaryotic translation initiation factor 4E-binding protein; FoxO1, forkhead box O1; mTOR, mammalian target of rapamycin; p-, phosphorylated; Pras40, proline-rich AKT substrate of 40 kDa; Rictor, rapamycin-insensitive companion of mTOR; Rps6, ribosomal protein S6; t-, total.