KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Rabbit polyclonal anti-UCP1 | Abcam | ab10983; RRID: AB_2241462 |
| Chicken polyclonal anti-GFP | Abcam | ab13970; RRID: AB_330798 |
| Rabbit polyclonal anti-TUBB3 | BioLegend | 802001; RRID: AB_2564645 |
| Rabbit polyclonal anti-Recoverin | Millipore Sigma | AB5585; RRID: AB_2253622 |
| Phalloidin (Alexa Fluor 594) | Thermo Fisher Scientific | A12381; RRID: AB_2315633 |
| Mouse monoclonal anti-Rhodopsin | Abcam | ab3267; RRID: AB_303655 |
| Isolectin IB4 (Alexa Fluor 488) | Thermo Fisher Scientific | I21411; RRID: AB_2314662 |
| Anti-Phospho-PKA (pThr197) | Cell Signaling Technology | 5661S; RRID: AB_10707163 |
| Anti-PKA C-a | Cell Signaling Technology | 5842S; RRID: AB_10706172 |
| Anti-Phospho-PKA Substrate (RRxpS/T) | Cell Signaling Technology | 9624S; RRID: AB_331817 |
| Anti-Phospho-HSL (pSer660) | Cell Signaling Technology | 4126S; RRID: AB_490997 |
| Anti-HSL | Cell Signaling Technology | 18381T; RRID: AB_2798800 |
| Anti-Phospho-PLIN1 (pSer522) | Vala Sciences | 4856 |
| Anti-Phospho-CREB (pSer133) | Cell Signaling Technology | 9198S; RRID: AB_2561044 |
| Anti-phospho-ERK (p44/42) | Cell Signaling Technology | 9102S; RRID: AB_330744 |
| Chemicals, Peptides, and Recombinant Proteins | ||
| DMEM High Glucose | Thermo Fisher Scientific | 11965–092 |
| Insulin | Millipore Sigma | I2643 |
| Rosiglitazone | Millipore Sigma | R2408 |
| Dexamethasone | Millipore Sigma | D1756 |
| IBMX | Millipore Sigma | I5879 |
| 9-cis-Retinal | Millipore Sigma | R5754 |
| Critical Commercial Assays | ||
| cAMP Assay Kit (Competitive ELISA, Fluorometric) | Abcam | ab138880 |
| OxPhos Rodent WB Antibody Cocktail | Thermo Fisher Scientific | 45–8099; RRID: AB_2533835 |
| Free Glycerol Reagent | Millipore Sigma | F6428 |
| GeneJET RNA Purification Kit | Thermo Fisher Scientific | K0732 |
| Verso cDNA Synthesis Kit | Thermo Fisher Scientific | AB1453 |
| NAD/NADH Assay Kit (Colorimetric) | Abcam | ab65348 |
| Deposited Data | ||
| Gene Expression Dataset | GEO NCBI | GEO: GSE140757 |
| Experimental Models: Organisms/Strains | ||
| Mouse: Adipoq-cre: B6.FVB-Tg(Adipoq-cre)1Evdr/J | The Jackson Laboratory | 010803; RRID: IMSR_JAX:010803 |
| Mouse: Ucp1-cre: B6.FVB-Tg(Ucp1-cm)1Evd/J | The Jackson Laboratory | 024670; RRID: IMSR_JAX:024670 |
| Mouse: Ai14: B6;129S6-Gt(ROSA)26Sortm14(CAG-tdTomato)Hze/J | The Jackson Laboratory | 007908; RRID: IMSR_JAX:007908 |
| Mouse: Opn2: B6.129S6(Cg)-Rhotm1.1Kpal/J | The Jackson Laboratory | 017628; RRID: IMSR_JAX:017628 |
| Mouse: C57BL/6J | The Jackson Laboratory | 000664; RRID: IMSR_JAX:000664 |
| Mouse: Rx-cre: B6.Cg-Tg(Rax-cre)1Zkoz/Ph/J | Klimova et al., 2013 | N/A |
| Mouse: Opn3lacZ/lacZ | This paper | N/A |
| Mouse: Opn3fl/fl | This paper | N/A |
| Mouse: Opn3ΔEx2/ΔEx2 | This paper | N/A |
| Mouse: Opn3cre | This paper | N/A |
| Mouse: Opn3-eGFP: B6-Tg(Opn3-EGFPJY3Gsat/Mmucd)/J | GENSAT | 030727-UCD; RRID: MMRRC_030727-UCD |
| Mouse: Opn4: Opn4tm1Skay | KOMP Repository | MGI: 2449695 |
| Mouse: Opn3: Opn3tm2a(EUCOMM)Wtsi | EuMMCR Repository | MGI: 4434308 |
| Mouse: Opn4cre; Z/EG | Ecker et al., 2010 | N/A |
| Oligonucleotides | ||
| Dio2 F: CAGTGTGGTGCACGTCTCCAATC | This Paper | N/A |
| Dio2 R: TGAACCAAAGTTGACCACCAG | This Paper | N/A |
| Prdm16 F: CAGCACGGTGAAGCCATTC | This Paper | N/A |
| Prdm16 R: GCGTGCATCCGCTTGTG | This Paper | N/A |
| Pgc1α F: CCCTGCCATTGTTAAGACC | This Paper | N/A |
| Pgc1α R: TGCTGCTGTTCCTGTTTTC | This Paper | N/A |
| Cidea F: TGCTCTTCTGTATCGCCCAGT | This Paper | N/A |
| Cidea R: GCCGTGTTAAGGAATCTGCTG | This Paper | N/A |
| Ucp1 F: ACTGCCACACCTCCAGTCATT | This Paper | N/A |
| Ucp1 R: CTTTGCCTCACTCAGGATTGG | This Paper | N/A |
| Pparγ F: GTGCCAGTTTCGATCCGTAGA | This Paper | N/A |
| Pparγ R: GGCCAGCATCGTGTAGATGA | This Paper | N/A |
| Hprt1 F: TCAGTCAACGGGGGACATAAA | This Paper | N/A |
| Hprt1 R: GGGGCTGTACTGCTTAACCAG | This Paper | N/A |
| Tbp F: GAAGCTGCGGTACAATTCCAG | This Paper | N/A |
| Tbp R: CCCCTTGTACCCTTCACCAAT | This Paper | N/A |
| Software and Algorithms | ||
| GraphPad Prism 8.2.1 | GraphPad | GraphPad.com |
| MATLAB 2018a | Mathworks | Mathworks.com |
| ImageJ/Fiji 2.0.0 | ImageJ | ImageJ.nih.gov |
| AltAnalyze | Salomonis, 2012 | AltAnalyze.org |
| FLIR Tools Version 2.1 | FLIR | Flir.com |
| Other | ||
| Infrared Thermal Camera | FLIR | T530 |
| Rectal Thermometer Probe | Physitemp | RET-3 |
| TSE PhenoMaster | TSE Systems Inc. | N/A |
| JAZ Spectrometer | Ocean Optics | JAZ |