Adaptive Thermogenesis in Mice Is Enhanced by Opsin 3-Dependent Adipocyte Light Sensing

. Author manuscript; available in PMC: 2020 Jul 8.

Published in final edited form as: Cell Rep. 2020 Jan 21;30(3):672–686.e8. doi: 10.1016/j.celrep.2019.12.043

Allele	Primers	Pairs	bp
Opn3	F1: ACCCAGGCTTCTTTTGGTCT	F1R1 – Wild-type	1191
	R1: AGAGTCGTTGGCATCCTTGG	F1R1 – Opn3^fl	1231
	F2: ACTATCCCGACCGCCTTACT	F1R2 – Opn3^fl	1610
	R2: GAACTGATGGCGAGCTCAGA	F1R2 – Opn3^cko	640
		F2R2 – Opn3^lacz	701
Opn3^cre	F1: TGCTGGCCTATGAACGTTATATCC	F1R1 – Wild-type	401
	R1: TCAGTTCTGGGTGACTAACTGATC	F1R2 – Opn3^cre	390
	R2: CACTCGTTGCATCGACCGGTAATGC
Adipoq^cre	F: GCATTACCGGTCGATGCAACGAGTGATGAG	FR – cre	450
Ucp1^cre	R: GAGTGAACGAACCTGGTCGAAATCAGTGCG
Rx^cre
Opn3^ΔEx2	F1: CTCAGAACCCACAAAGTGCTGG	F1R1 – Wild-type	307
	R1: GTGGACTGCAATGTCCCATCTATC	F1R2 – Opn3^ΔEx2	191
	R2: GTGGGCATCATAGCCCATTGCTAC
Opn4	F1: AGGAGTGTATAGAGCCGGAAGTCTG	F1R1 – Wild-type	520
	R1: CCAGTCCAGAAGCCTAGGGCATGCC	F2R2 – Opn4⁻	380
	F2: TGCTCCTGCCGAGTATCCATCATGGC
	R2: CGCCAAGCTCTTCATATCACGGGTAG
Opn4^cre	F1: AGGCTGGATGGATGAGAGC	F1R1 – Wild-type	187
	R1: GTTGTGAAGCTGGGATCCTG	F2R2 – cre	184
	F2: CGACCAGGTTCGTTCACTCA
	R2: CAGCGTTTTCGTTCTGCCAA
Z/EG	F: CCCCTGCTGTCCATTCCTTA	FR – Z/EG	224
Z/EG	R: TGACCATGATTACGCAAGC