REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Donkey anti-mouse RRX	Jackson Immuno Research	115-295-003; RRID: AB_2338756
Goat anti-mouse Alexa 488	Life Technologies	A11029; RRID: AB_138404
Goat anti-rabbit Alexa 555	Abcam	ab150086; RRID: AB_2722519
Goat anti-rabbit Alexa 488	Life Technologies	A11034; RRID: AB_2576217
Mouse anti‐Engrailed	Developmental Studies Hybridoma Bank	4D9; RRID: AB_528224
Rabbit anti‐Diaphanous	[34]	N/A
Rabbit anti-pMRLC	Cell Signaling	3671S; RRID: AB_330248
Mouse anti-PH3	Millipore	3H10 05-806; RRID: AB_310016
Rabbit anti-PH3	Millipore	3H10 06-570; RRID: AB_1163440
Chemicals, Peptides, and Recombinant Proteins
DAPI	Sigma-Aldrich	D8417
Fluoromount G slide mounting medium	SouthernBiotech	0100-01
Phalloidin Alexa-647	Life Technologies	A22287
Cell-Tak	Corning	354240
Shields and Sang M3 media	Sigma	S3652
Fetal Bovine Serum	Sigma	F4135
Penicillin-Streptomycin (10,000 U/mL)	Life Technologies	15140-122
20-Hydroxyecdysone	Sigma	H5142
Insulin solution from bovine pancreas, 10mg/mL	Sigma	I0516-5ML
16% w/v Formaldehyde	TAAB Laboratories	F017/3
GelPak, 6.5 mil	GelPak	PF-70-x4/6.5mil
Microposit EC solvent	Dow Chemicals	N/A
Su-8 2050	Microchem	Su-8 2050
SYLGARD 184 elastomer kit	Dow Corning	1673921
Triton X-100	Sigma	T8787
Bovine Serum Albumin (BSA)	Sigma	A7030
Experimental Models: Organisms/Strains
yw;;;	BDSC	N/A
;;SqhGFP, HisRFP;	Y. Mao	N/A
;actGal4/CyO;;	B. Baum	N/A
;trol-RNAi;;	VDRC	GD/24549
UAS-trol;;;	Kyoto	DGRC 201233
;act > flp > Gal4, UAS-GFP; His-RFP;	Y. Mao	N/A
ywhsflp; Rok-RNAi;;	Y. Mao & VDRC	KK/104675
ywhsflp; dia-RNAi;;	Y. Mao & VDRC	KK/103914
;Bsg-GFP, His-RFP;;	Y. Mao & Kyoto	DGRC 115366
;EnGal4, ECad-GFP; UAS-NLS-Cherry;	Y. Mao & B. Sanson	N/A
;Rok-RNAi;;	VDRC	KK/104675
;dia-RNAi;;	VDRC	KK/103914
;enGal4; SqhGFP, HisRFP;	Y. Mao & B. Sanson	N/A
;nubGal4; UAS-His2A-DsRed;	B. Thompson	N/A
Software and Algorithms
GraphPad Prism 7	GraphPad Software	N/A
Fiji	[35]	N/A
Microsoft Excel 2011	Microsoft	N/A
MATLAB 2016	MathWorks	N/A