REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Mouse monoclonal anti-HLA-DR (cloneL243)	Sigma-Aldrich	Cat#SAB4700731
Mouse monoclonal anti-HLA-DR alpha chain (clone TAL.1B5)	DAKO	Cat#M0746; RRID: AB_2262753
Mouse monoclonal anti-DnaK (clone 8E2/2)	ENZO	Cat#ADI-SPA-880-F; RRID: AB_10619012
Goat polyclonal anti-Common Salmonella Antigens	KPL	Cat#01-91-99
Rabbit Monoclonal anti-GFP	Invitrogen	Cat#G10362
Mouse monoclonal anti anti-ubiquitin (clone P4D1)	Santa Cruz	Cat#sc-8017; RRID: AB_628423
Mouse monoclonal anti-tubulin	DSHB	Cat#E7
Rabbit polyclonal anti-actin	Sigma-Aldrich	Cat#A2066; RRID:AB_476693
Mouse monoclonal anti-CRISPR-Cas9	Novus Biological	Cat#NBP2-36440
Mouse monoclonal anti-Golgin97 (clone CDF4)	eBioscience	Cat#14-9767-82
Mouse monoclonal anti-Transferrin receptor (H68.4)	Zymed (S. Meresse)	Cat#13-6800
Mouse monoclonal anti-FLAG (clone M2)	Merck	Cat#F3165-2MG
Rabbit polyclonal anti-FLAG	Merck	Cat#F7425
Mouse monoclonal anti-HA11 (clone 16B12)	Biolegend	Cat#901502; RRID: AB_2565007
Rabbit polyclonal anti-TMEM127	Bethyl laboratories	Cat#A303-450A; RRID: AB_10952702
Rabbit polyclonal anti-WWP2	Abcam	Cat#ab103527; RRID: AB_10710285
Mouse monoclonal anti-MHCII-APC (clone M5/114.15.2)	Miltenyi Biotec	Cat#130-102-898; RRID: AB_2660057
Hamster monoclonal anti-CD11c-VioBlue (clone N418)	Miltenyi Biotec	Cat#130-102-797; RRID: AB_2660157
Rat monoclonal anti-CD4-PerCP-Cy5.5 (clone RM4-5)	BD Biosciences	Cat#550954; RRID: AB_393977
Hamster monoclonal anti-CD3ε-PE-Vio770 (145-2C11)	Miltenyi Biotec	Cat#130-102-794; RRID: AB_2660399
Bacterial and Virus Strains
Salmonella enterica serovar Typhimurium 12023	NCTC	NCTC 12023
Salmonella enterica serovar Typhimurium 12023 ΔsteD::km	Bayer-Santos et al., 2016	N/A
Salmonella enterica serovar Typhimurium 12023 ΔsteD::km + pSteD-2HA-SrcA	Godlee et al., 2019	N/A
Salmonella enterica serovar Typhimurium 12023 + pFCcGi	This study	N/A
Salmonella enterica serovar Typhimurium 12023 ΔsteD::km + pFCcGi	This study	N/A
Salmonella enterica serovar Typhimurium 12023 ΔsteD::km + pSteD-2HA-SrcA + pFCcGi	This study	N/A
Salmonella enterica serovar Typhimurium 12023 ΔsteD::km + pSteDK24R-2HA-SrcA	This study	N/A
Salmonella enterica serovar Typhimurium 12023 ΔsteD::SteDK24R	This study	N/A
E. coli DH5α	Thermo Fisher Scientific	Cat#18265017
Chemicals, Peptides, and Recombinant Proteins
Chloroquine diphosphate salt	Sigma-Aldrich	Cat#C6628-25G; CAS: 50-63-5
Enzymes for UbiCRest	Hospenthal et al., 2015	N/A
Blasticidin S	Merck	Cat#15205-25MG; CAS: 3513-03-9
Puromycin dihydrochloride	Sigma-Aldrich	Cat#P8833-25MG; CAS: 58-58-2
Carbenicillin disodium salt	Sigma-Aldrich	Cat#C1389-5g; CAS: 4800-94-6
Chloramphenicol	Merck	Cat#C0378-25G; CAS: 56-75-7
Kanamycin sulfate from Streptomyces kanamyceticus	Sigma-Aldrich	Cat#K1377-5g; CAS: 25389-94-0
5(6)-Carboxyfluorescein diacetate N-succinimidyl ester	Sigam-Aldrich	Cat#21888-25MG-F; CAS: 150347-59-4
Ovalbumin (323-339) (chicken, Japanese quail)	Sigam-Aldrich	Cat#O1641-1MG
Experimental Models: Cell Lines
Hek293T	Provided by F. Randow	N/A; RRID: CVCL_0063
Hybridoma L243	Provided by S. Meresse	N/A; RRID: CVCL_4533
MelJuSo	Provided by J. Neefjes	N/A; RRID: CVCL_1403
MelJuSo CRISPR/Cas9 MARCH8 KO	This study	N/A
MelJuSo CRISPR/Cas9 TMEM127 KO	This study	N/A
MelJuSo CRISPR/Cas9 WWP2 KO	This study	N/A
MutuDCs	Provided by A. Orbea	Fuertes Marraco et al., 2012
MutuDCs CRISPR/Cas9 MARCH1 KO	Provided by J. Mintern	Wilson et al., 2018
MutuDCs CRISR/Cas9 TMEM127 KO	This study	N/A
MutuDCs CRISR/Cas9 WWP2 KO	This study	N/A
Experimental Models: Organisms/Strains
OT-II mice	Charles River	C57BL/6-Tg(TcraTcrb)425Cbn/Crl; RRID: IMSR_CRL:643
Oligonucleotides
See Table S2 for primer sequences	N/A	N/A
Recombinant DNA
Plasmid: GFP-SteD	Bayer-Santos et al, 2016	N/A
Plasmid: pMD-GAGPOL	Provided by F. Randow	Randow and Sale, 2006
Plasmid: VSVG	Provided by F. Randow	Randow and Sale, 2006
Plasmid: lentiCas9-Blast	Sanjana et al, 2014 Gift from Feng Zeng	Addgene Plasmid #52962
Plasmid: psPAX2	Gift from Didier Trono	Addgene Plasmid #12260
Plasmid: pMD2.G	Gift from Didier Trono	Addgene Plasmid #12259
Plasmid: lentiguide-puro	Sanjana et al, 2014 Gift from Feng Zhang	Addgene Plasmid #52963
Pooled plasmid library: Human CRISPR knockout Pooled Library (GeCKO v2)	Sanjana et al., 2014 Gift from Feng Zhang	Addgene Plasmid #1000000048
WWP2 gene sequence	Genscript	OHu14116
TMEM127 gene sequence	genscript	OHu10702
Software and Algorithms
Biopython	Biopython	https://biopython.org
Count_spacers.py	Sanjana et al, 2014	https://github.com/fengzhanglab/Screening_Protocols_manuscript/blob/master/count_spacers.py
CRISPRAnalyzer	CRISPRAnalyzeR	http://crispr-analyzer.dkfz.de/
CRISPR Design Tool	Dharmacon	https://horizondiscovery.com/en/products/tools/CRISPR-Design-Tool
Prism v8	GraphPad	https://www.graphpad.com/scientific-software/prism/
FIJI	ImageJ	https://fiji.sc/
Coloc 2 ImageJ plugin	ImageJ	http://imagej.net/Coloc_2
Image Lab	BioRad	https://www.bio-rad.com/en-uk/product/image-lab-software?ID=KRE6P5E8Z
FlowJo v10	BD Life Sciences	https://www.flowjo.com/
Other
Laser Scanning Microscope 710	Zeiss	N/A
BD LSRFortessa™	BD Biosciences	N/A
BD FACSAria™ III	BD Biosciences	N/A
Dionex Ultimate 3000 HPLC system	Thermo Fisher Scientific	N/A
Q Exactive™	Thermo Fisher Scientific	N/A