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. 2020 Jul 1;11:1238. doi: 10.3389/fimmu.2020.01238

Figure 5.

Figure 5

Effects of aqueous extract of A. brasiliensis on oxidative and genotoxic parameters of septic mice. (A) ROS production by peritoneal cells collected from septic animals 24 h after CLP induction. (B) ROS production by thioglycollate-elicited peritoneal macrophages after in vitro incubation with tBHP or tBHP plus Ab (22.5 mg/mL) (C) Tail length (μm), (D) DNA tail (%), (E) Tail Moment (TM), (F) Olive Moment (OM) in peripheral blood mononuclear cells from healthy volunteers incubated in vitro with tBHP or tBHP plus A. brasiliensis. (G) Representative images of comet assay of human cells incubated in RPMI medium, RPMI plus tBHP and tBHP plus Ab, (H) MDA levels in serum, (I) MDA levels in peritoneal cavity, and (J) MDA levels in heart and liver at 12 and 24 h after CLP. The results were expressed as the mean ± SD. (*p < 0.05 Saline-CLP or Ab-CLP vs. Sham; #p < 0.05 Ab-CLP vs. saline-CLP).