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| 引物名称 | 正义引物P1（5′→3′） | 反义引物P2（5′→3′） | 产物大小（bp） |
| SH2 | CGGGGTACCGCCACCATGTGGTTTTTTGGCAAAATCCCCAG | ACGCGATATCTTCTATGTCCCGCAGGAATATCTG | 294 |
| DED-HA | CGGGGTACCGCCACCATGCCGTTCCTGGTGCTGCTGCAC | CCCAAGCTTCTAAGCGTAGTCTGGGACGTCGTATGGGTActcGTCGTCGACGCGCCGCAGCAGGT | 306 |
| 拼接Linker | SH2-Linker-P2:ACCACCACCAGAACCACCACCACCTTCTATGTCCCGCAGGAATATCTG | DED-Linker-P1:GGTGGTTCTGGTGGTGGTGGTTCTCCGTTCCTGGTGCTGCTGCAC | 308 |
| Sm- R86K-L | CTCTCACTCTCTTTGATAAGAAAG | CTTTCTTATCAAAGAGAGTGAGAG | 308 |
