Table 2.
Yield, Purity, and Mass Spectrometry for Synthetic Peptidesa
| peptide | yield (nmol) | % purity | calculated M+ Da | observed M+ Da |
|---|---|---|---|---|
| [Asn9]PeIA | 5569 | >98 | 1678.63 | 1678.65 |
| [Tyr9]PeIA | 3714 | >98 | 1727.65 | 1727.75 |
| [Thr9]PeIA | 3541 | >97 | 1665.64 | 1665.36 |
| [Ile10]PeIA | 2714 | >98 | 1665.54 | 1665.72 |
| [Nle10]PeIA | 3643 | >98 | 1665.54 | 1665.69 |
| [Thr10]PeIA | 2742 | >99 | 1653.60 | 1653.57 |
| [Asp11]PeIA | 5311 | >97 | 1652.61 | 1652.69 |
| [Aadn]PeIA | 2591 | >98 | 1680.63 | 1680.63 |
| [Api11]PeIA | 1637 | >98 | 1694.63 | 1694.82 |
| [Asu11]PeIA | 3957 | >98 | 1708.63 | 1708.68 |
| [Gla14]PeIA | 2014 | >98 | 1695.61 | 1695.62 |
| [Asp14]PeIA | 3440 | >98 | 1637.61 | 1637.65 |
| [Adi14]PeIA | 2244 | >97 | 1665.63 | 1665.66 |
| [Asu14]PeIA | 1809 | >97 | 1693.63 | 1693.68 |
| [Gln14]PeIA | 2571 | >98 | 1650.64 | 1650.63 |
| [Arg14]PeIA | 1650 | >98 | 1678.68 | 1678.80 |
| [Arg15]PeIA | 2027 | >98 | 1694.64 | 1694.79 |
| [Val15]PeIA | 1951 | >98 | 1637.61 | 1637.66 |
| [Ile15]PeIA | 3749 | >99 | 1651.62 | 1651.67 |
| [Nle15]PeIA | 2774 | >98 | 1651.62 | 1651.62 |
| PeIA-4769 | 5289 | >98 | 1715.67 | 1715.69 |
| PeIA-5106 | 5493 | >96 | 1715.67 | 1715.53 |
| PeIA-5115 | 7226 | >97 | 1716.65 | 1716.75 |
| PeIA-5080 | 1145 | >96 | 1730.67 | 1730.81 |
| PeIA-5415 | 2842 | >98 | 1744.67 | 1744.80 |
| PeIA-5355 | 1292 | >98 | 1758.67 | 1758.65 |
| PeIA-5587 | 3644 | >97 | 1772.67 | 1772.78 |
| PeIA-5413 | 3753 | >98 | 1758.67 | 1758.76 |
| PeIA-5416 | 2523 | >98 | 1758.67 | 1758.82 |
| PeIA-5466 | 2603 | >98 | 1758.67 | 1758.84 |
a
MALDI-TOF mass spectrometry was used to determine the masses of all peptides except for [Gla14]PeIA, which was determined using ESI mass spectrometry. RP-HPLC chromatograms for each of the peptides showing the purity are presented in the SI Figure 2.