Table 3.
The Information of Antibodies
| Gene Name | Catalog Number | Company | Diluted Concentration | Secondary Antibody |
|---|---|---|---|---|
| β-actin | A4552 | Sigma | 1:20,000 | Mouse |
| RRM2 | ab57653 | Abcam | 1:500 | Mouse |
| Akt | 4691 | Cell Signaling Technology | 1:1000 | Rabbit |
| mTOR | 2983T | Cell Signaling Technology | 1:1000 | Rabbit |
| 4EBP1 | 9644 | Cell Signaling Technology | 1:1000 | Rabbit |
| PRAS40 | 2691 | Cell Signaling Technology | 1:1000 | Rabbit |
| Phospho-Akt | 4060 | Cell Signaling Technology | 1:2000 | Rabbit |
| Phospho-mTOR | 5536 | Cell Signaling Technology | 1:1000 | Rabbit |
| Phospho-PRAS40 | 2997 | Cell Signaling Technology | 1:1000 | Rabbit |
| Phospho-4EBP1 | 2855 | Cell Signaling Technology | 1:1000 | Rabbit |
| Anti-mouse IgG | 7076 | Cell Signaling Technology | 1:5000 | |
| Anti-rabbit IgG | 7074 | Cell Signaling Technology | 1:5000 |