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. 2020 Jun 28;26:e924564-1–e924564-11. doi: 10.12659/MSM.924564

Figure 1.

Figure 1

The expression of METRNL was downregulated in H9C2 cells during OGD/R, and METRNL overexpression inhibited the inflammatory cytokines secretion. (A, D) The expression of METRNL in H9C2 cells was determined by western blot. (B, E) Relative expression of METRNL in H9C2 cells was evaluated by qRT-PCR. (C) The viability of H9C2 cells was analyzed by CCK8 assay. (F) The levels of inflammatory cytokines including TNF-α, IL-1β, and IL-6 were measured by corresponding ELISA kits. Error bars represent the mean±SEM from 3 independent experiments. * P < 0.05, ** P<0.01, *** P<0.001 versus Control; ## P<0.01, ### P<0.001 versus OGD/R 12 hours. METRNL – Meteorin-like; OGD/R – oxygen-glucose deprivation and reperfusion; qRT-PCR – quantitative real-time polymerase chain reaction; CCK-8 – Cell Counting Kit-8; TNF – tumor necrosis factor; IL – interleukin; ELISA – enzyme-linked immunosorbent assay; SEM – standard error of the mean.