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. 2020 Jun 28;26:e924564-1–e924564-11. doi: 10.12659/MSM.924564

Figure 5.

Figure 5

PAK2 silencing blocked the inhibitive effect of METRNL overexpression on cell apoptosis induced by OGD/R. (A) The apoptosis rate of H9C2 cells was analyzed by TUNEL assay (200x magnification). (B) The expressions of Bcl2, Bax, cleaved caspase-3, pre caspase-3, caspase-12, and PARP were evaluated by western blot. Error bars represent the mean±SEM from 3 independent experiments. *** P<0.001 versus Control; ## P<0.01, ### P<0.001 versus OGD/R 12 hours; @ P<0.05, @@ P<0.01 versus OGD/R+Ov-METRNL. PAK2 – p21-activated kinase 2; METRNL – Meteorin-like; OGD/R – oxygen-glucose deprivation and reperfusion; TUNEL – terminal deoxynucleotidyl transferase dUTP nick end labeling immunohistochemical; PARP – poly ADP-ribose polymerase; SEM – standard error of the mean; Ov-METRNL – overexpression-METRNL.