Figure 4.

CD mice have more severe contextual fear phenotypes than double mutants. (A) The conditioned fear task design. Day 1 animals are delivered a tone and then a footshock throughout the 5 min task. Day 2 the animals are put in the same context without a footshock to measure contextual fear memory. Day 3 animals are put in a new chamber and delivered the tone to measure cued fear memory. (B) Percent time freezing during conditioned fear acquisition. CD mice have increased baseline freezing during Minute 1 and Gtf2i^* mutants show decreased freezing during Minute 5. (C) Percent time freezing during contextual fear memory recall. CD mice show elevated freezing during fear memory recall. (D) Percent time freezing during cued fear memory recall. All animals show increased freezing when the tone is played. ^*P < 0.05, ^**P < 0.01, ^***P < 0.001.