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. 2020 Jul 8;15(7):e0235033. doi: 10.1371/journal.pone.0235033

Fig 5. Normalized β-galactosidase activity is lower in yta6Δ and ypr096cΔ for structured mRNAs.

Fig 5

A strong hairpin structure (p281-4) (A) upstream of a LacZ reporter pTAR (C) highly structured 5’-UTR of HIV1-tar and pRTN (D) constructs contain the highly structured 5’-UTR of FOAP-11 genes in front of the β-galactosidase reporter mRNA. P281 (B) was served as a control plasmid with no inhibitory structure did not show as significant reductions in β-galactosidase activity. Values are normalized to that for WT which resulted in average β-galactosidase values of 14.1U and 37.9U for pRTN and p416 constructs, respectively. Each experiment was repeated at least three times (n ≥ 3) and error bars represent standard deviation. * represent statistically significant results (P-value ≤ 0.05) compared to the WT. t-test analysis (P-value ≤ 0.05) was used to compare differences. The insets represent schematic reporter mRNA structures.