Fig 2. JNK pathway regulates ROS metabolism.

Effect of JNK (A) and Puc (B) silence on the expression of antioxidant genes: OXR1, Cat, GPX and Prx1 of the pea aphids. The expressions of OXR1, Cat, GPX and Prx1 were normalized with rpl7 of the pea aphids. The statistical differences between the dsGFP injected groups and dsJNK or dsPuc injected groups were denoted with asterisks. Effect of JNK (C) and Puc (D) silence on the H₂O₂ concentration in the aphids uninfected and infected by M. luteus (M.l) and P. aeruginosa (P.a). Ten aphids from each group at each time point were used for the measurements of H₂O₂ concentration. The statistical differences between the compared groups were denoted with asterisks. For (A-D), the values shown are the mean (±SEM) of three independent experiments. P values were determined by Student’s t test. *P<0.05; **P<0.01; ***P<0.001.