Fig 7. miRNA-184a/b affected pea aphid survival and bacteria multiplication after infection.

(A) Effect of injection of agomir-184a, agomir-184b and half-dose agomir-184a plus half-dose agomir-184b on the survival of pea aphids after M. luteus (M.l) and P. aeruginosa (P.a) infection with the aphids injected by sterile 0.85% as control groups, n = 20. Survival graphs show one representative experiment out of three independent experiments with similar results. The statistical differences between the compared groups were denoted with asterisks. The log-rank (Mantel-Cox) test was used to analyze pea aphids’ survival curves. *P<0.05. (B) Effect of injection of agomir-184a, agomir-184b and half-dose agomir-184a plus half-dose agomir-184b on the bacterial load of pea aphids after M. luteus (M.l) and P. aeruginosa (P.a) infection, n = 8. Each dot in the graph represents an individual aphid. The horizontal bars indicate mean values and the vertical bars indicate the SEM of the replicates. The statistical differences between the compared groups were denoted with asterisks. P values were determined by Student’s t test. *P<0.05.