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SBW25 WT or ΔrimK cells were grown in either M9-pyruvate (abbreviated M9-Pyr) or M9-pyruvate with Cas-aminoacids (abbreviated M9-Pyr-CAS) to either early exponential phase or stationary phase as indicated. Each panel shows two biological duplicates; in each case the 12KDa molecular weight marker is shown (Hfq-FLAG has a predicted molecular weight of 12.04 KDa).
