qRT-PCR analysis of IL-6, IL-10, CD86, Btk, and CD163 revealed an inflammatory state in the brains of RSA59-infected CD4−/− mice. Quantitative-PCR analysis of IL-6 (a), IL-10 (b), CD86 (c), Btk (d), and CD163 (e) was performed in RSA59-infected CD4+/+ and CD4−/− mouse brains at days 5, 10, and 15. The data represent the results from 3 independent biological experiments, with 3 technical replicates each. Statistical analysis was performed by two-way ANOVA and the Holm-Sidak multiple-comparison test; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001. The error bars represent SEM.