FIG 5.

Absence of CD4 resulted in a significant reduction of CD11b-positive microglia/macrophages in brains and spinal cords during RSA59-induced acute infection. (Left) At day 6 p.i., sections of brains and spinal cords from CD4^+/+ mice and CD4^−/− mice were immunohistochemically stained for CD11b (macrophage/microglia activation marker). The boxed areas are shown at higher magnification below the corresponding brain midsagittal sections (top) or cross sections of spinal cord (bottom). The arrows mark microglia/macrophages in the in the CD11b immunohistochemically stained sections. (Right) Quantification of the intensity of staining plotted in a scatter diagram. Statistical analysis was performed using Student's t test and Welch correction. **, P < 0.01; ****, P < 0.0001. The data are presented as means and SEM from 5 independent biological experiments.