FIG 3.

PDCoV preferably infects the ileum and jejunum over the duodenum and colon in vivo. (A) Detection of PDCoV antigens by an immunohistochemistry assay. SPF piglets were orally inoculated with 1 ml 1 × 10⁴ TCID₅₀ PDCoV or DMEM. Intestinal tissue samples were collected at 72 hpi and stained with a mouse anti-PDCoV N protein monoclonal antibody. Scale bar, 100 μm. (B) Histological analysis of PDCoV infection by H&E staining. Intestinal sections were collected as indicated in panel A. Scale bar, 200 μm.