FIG 4.

Enteroids isolated from different intestinal segments display unique gene expression profiles. (A) Venn diagram of common differentially expressed genes of enteroids derived from jejunal, duodenal, and colonic crypts. Monolayers of jejunal enteroids (J_enteroids), duodenal enteroids (D_enteroids), and colonoids (C_ enteroids) isolated from three individual SPF 2-day-old piglets were cultured for 24 h. Total RNA of enteroids was extracted and sequenced by RNA-seq. (B) Heat map comparing gene expression levels among enteroids derived from jejunal, duodenal, and colonic crypts. (C) Viral gene transcript levels of PDCoV-infected enteroids derived from jejunal, duodenal, and colonic crypts were measured by RNA-seq. Monolayers of jejunal enteroids, duodenal enteroids, and colonoids were infected with PDCoV at an MOI of 2. Total RNA was isolated at 24 hpi and used for RNA-seq. The transcript levels of viral genes were shown as log(FPKM) values. (D) Venn diagram of common differential gene expression of PDCoV-infected enteroids derived from jejunal, duodenal, and colonic crypts. (E) Heat map of differentially expressed genes in PDCoV-infected enteroids derived from jejunal, duodenal, and colonic crypts. Data in panels B and E are based on log(RPKM) values from three independent enteroid preparations. J_PDCoV or mock, D_PDCoV or mock, and C_PDCoV or mock are shown as jejunal enteroids, duodenal enteroids, and colonoids infected with or without PDCoV, respectively.