FIG 6.

IFN receptor expression is not related to the differential infectivity of PDCoV in different intestinal segments. (A) Heat map of the IFN receptors expression in enteroids. Enteroid monolayers derived from jejunal, duodenal, and colonic crypts were infected with or without PDCoV for 24 h. Total RNA was isolated from enteroids, and the expression levels of IFN receptors were determined by RNA-seq. Data are based on log(RPKM) values from three independent enteroid preparations. (B to G) Verification of the IFN receptors expression in enteroids by RT-qPCR. Monolayers of jejunal enteroids (J_enteroids), duodenal enteroids (D_enteroids), and colonoids were cultured for 24 h. The mRNA expression levels of type I IFN receptors (B and C), type II IFN receptors (D and E), and type III IFN receptors (F and G) were measured by RT-qPCR. Data represent the means ±SEMs of results from three wells and three independent enteroid preparations. Error bars denote deviations. *, P < 0.05; ***, P < 0.005; ns, not significant.