FIG 7.

IFN responses are not related to the differential infectivity of PDCoV in different intestinal segments. (A) Heat map of highly upregulated ISGs expression in PDCoV-infected enteroids. Enteroid monolayers derived from jejunal, duodenal, and colonic crypts were infected with or without PDCoV for 24 h. The differential expression profiles of ISGs were determined by RNA-seq. Data are based on log(RPKM) values from three independent enteroid preparations. (B to G) Confirmation of ISGs expression in PDCoV-infected enteroids by RT-qPCR. Monolayers of jejunal enteroids, duodenal enteroids, and colonoids were infected with or without PDCoV at an MOI of 2 for 24 h. The mRNA expression levels of ISG15 (B), RSAD2 (C), IFIT3 (D), OASL (E), Mx1 (F), and Mx2 (G) were quantified by RT-qPCR. The results are presented as the means ± SEMs from three wells and three independent enteroid preparations.