TABLE 1.
Surface plasmon resonance analysis of HAdV hexon interactions with hLF and hLfcin1-49
Ligand | Analyte | Parametera
|
||
---|---|---|---|---|
KD | ka (M−1 · s−1) | kd (s−1) | ||
HAdV-C5 hexon | hLF | 29 ± 3.6 nM | 5.3 × 104 ± 2.6 × 103 | 0.00154 ± 0.00024 |
HAdV-B35 hexon | hLF | 151 ± 14 nM | 2.5 × 104 ± 2.5 × 103 | 0.00354 ± 0.00014 |
HAdV-A31 hexon | hLF | 827 ± 55 nM | 1.2 × 104 ± 1.1 × 103 | 0.01011 ± 0.00024 |
HAdV-C5 hexon | hLfcin1-49 | 0.73 ± 0.206 μM | ND | ND |
HAdV-B35 hexon | hLfcin1-49 | 22.1 ± 13.6 μM | ND | ND |
HAdV-A31 hexon | hLfcin1-49 | 187 ± 155 μM | ND | ND |
Data are shown as averages from three independent measurements. KD, equilibrium dissociation rate constant; ka, association rate constant; kd, dissociation rate constant; ND, not determined due to very fast kinetics and likely large errors in determining kinetics.