TABLE 3.
Constructs and PCR primers used for construction
| Construct | Primer | Sequence (5′ → 3′)a |
|---|---|---|
| BVΔC | Bungo_dC_1012F | GACAAAGCCTAAAGAGACTGAACCAGAAGCGTCCAGGAAGAAG |
| Bungo_1499R | GATTGATCCACGGCTCTATATTG | |
| BVΔE2 | Bungo_2060F | CTGAGCTTAATTGCAATGTC |
| Bungo_dE2_R | GAGCCACTATGTTCTCGTCGGCTTGTGCTCCCCCTATG | |
| BVΔERNS_156 | Bungo_dErns_F | CATCTAGCAGCAGACTATGAAAGTAAGATTGAAAACACCAAGA |
| Bungo_2164R | CATCACGAAGTCCCTGTTGTC | |
| BVΔC-E2 | Ph_dCE2_Bungo _F | GACAAAGCCTAAAGAGACTGAATGGTTTGATCTCACAGCAAAG |
| Bungo_4021R | GAGACTTATTACAGCCACAAG | |
| BVΔERNS_55 | Ph_Bungo_dErns_55F | CAAGCATGGGTGGTGCAACAAGAAAGGGCACAACTTCTC |
| Bungo_2164R | CATCACGAAGTCCCTGTTGTC | |
| BVΔERNS_88 | Ph_Bungo_dErns_88F | CATCTAGCAGCAGACTATGAAAAGAAAGGGCACAACTTCTC |
| Bungo_2164R | CATCACGAAGTCCCTGTTGTC | |
| BVΔERNS_112 | Ph_Bungo_dErns_112F | CATCTAGCAGCAGACTATGAAGACTTGCTCAGGATCATGG |
| Bungo_2164R | CATCACGAAGTCCCTGTTGTC | |
| BVΔERNS | BV_dErns_kompF | GCCTATTGGTCGTGCCGGTAGGGTCCAGTCCTTACTGCCCAGTGGC |
| Bungo_2164R | CATCACGAAGTCCCTGTTGTC | |
| BVΔE1 | BVdE1A_F | CAGTCCTTACTGCCCAGTGGCTTTGTGGACCGAAGCAACAGC |
| Bungo_2874R | CTAGGGCATTCAAGTTCAAAGC | |
| BV_GAA_Nluc | Bungo_GAA_F | GATACATGTGTGTGGAGCTGCTGGTTTCTTGATAACTGAG |
| Bungo_11835R | CTGAGCCAATTTAGAGAAC | |
| pA/BV_Nluc | Ph_CNlucF | CTGACGACAAAGGAGCAAAAATGGTCTTCACACTCGAAGATTTC |
| Ph_NlucR | GAAGTTCGTGGCTCCGGAACCCGCCAGAATGCGTTCGCACAG | |
| pCAGGS_sigERNS_BV | CAGGS_sigErns_F | GGCAAAGAATTCGCCACCATGGAGGCCAGCCGGAAGAAGCTG |
| CAGGS_Erns_R | GAAAATAACATATGCGGCCGCTAGCTCAGGCCTCGGCGCCGAAG | |
| BVdERNS_2GcN | 2Gc_ F | CATCTAGCAGCAGACTATGAAAGTATTAACTGCAAAAACATT |
| Erns_GC_amino_R | CTTGGTGTTTTCAATCTTACTGATCAGAGATAAGGTAGTGAG | |
| BVdERNS_RBD | BV_dErns_RBD_F | CTAGCAGCAGACTATGAATCTTTCGAAGCAAAACCTTCTGG |
| BV_dErns_RBD_R | GGTGTTTTCAATCTTACTATATTCCACGCAATTGCCTAATTGAG | |
| BVdERNS_CSFV_E2 | BV_CSFV_E2_F | GGTCGTGCCGGTAGGGTCCCAGCTAGCCTGCAAGGAAG |
| BV_CSFV_E2_R | GCCACTGGGCAGTAAGGACTACCAGCGGCGAGTTGTTCT |
a
Restriction enzyme sites are underlined.