Table 2: Challenging beam performance in male and female mice at 4 months post-PFF injection.

Male and female mice (n = 10 per group) were tested on challenging beam 4 months after PFF injections. Two-way ANOVA with Sidak’s multiple comparison tests were performed. Results of Sidak post-tests between dieldrin exposed animals and corresponding vehicle controls are indicated on the table. All significant post-test results are included in this legend. Males:There were no significant differences in males on time to traverse. On steps, PFF, dieldrin and the interaction were a II statistically significant by two-way ANOVA (PFF: p = 0.0008; dieldrin, p < 0.0001; Interaction: p < 0.0001). Sidak post-tests shows a significant effect of dieldrin in saline-injected animals (vehicle:saline vs dieldrin:saline, p < 0.0001) but not in PFF-injected animals, as well as an effect of PFF in dieldrin exposed animals (dieldrin:saline vs dieldrin:PFF, p < 0.0001), but no effect of PFF in vehide animals. On errors/step, there was a significant effect of PFF by two-way ANOVA (PFF: p = 0.003; dieldrin, p = 0.1657; interaction, p = 0.0695). Sidak post-tests showed no significant effect of dieldrin, but did identify a PFF effect in dieldrin exposed animals (dieldrin:saline vs dieldrin:PFF, p = 0.0012). Females: There were no significant differences in time to traverse. On steps, PFF and the interaction were statistically significant (PFF: p = 0.0199; dieldrin, p = −.4907; interaction: p < 0.0001). Sidak post-tests revealed a significant effect on dieldrin in both saline-and PFF-injected animals (vehicle:saline vs dieldrin:saline, p = 0.0222; vehicle:PFFvs dieldrin: PFF, p = 0.0014), as well as a PFF-effect in vehicle exposed animals but not dieldrin exposed animals (vehicle:saline vs vehicle:PFF, p =< 0.0001; dieldrin:saline vs dieldrin: PFF, p =0.3510). On errors/step, PFF, dieldrin and the interaction were all statistically significant by two-way ANOVA (PFF: p = 0.0061; dieldrin, p = 0.0038; Interaction: p = 0.0048). Sidak post-tests revealed a significant effect of dieldrin in saline-injected animals (vehicle:saline vs dieldrin:saline, p = 0.0007), as well as a PFF-related increase in errors in vehicle animals (vehicle:saline vs vehide:PFF, p = 0.0011).

	Saline		PFF
Male	Vehicle	Dieldrin	Vehicle	Dieldrin
Time to traverse	11.136 (10.053-12.219)	9.462 (8.168-10.756)	10.849 (9.585-12.113)	10.863 (10.076-11.65)
Steps	16.84 (16.166-17.514)	11.760 (10.932-12.588)****	15.720 (14.933-16.507)	15.280 (14.625-15.935)
Errors/Step	0.080 (0.049-0.058)	0.116 (0.086-0.146)	0.057 (0.040-0.0674)	0.052 (0.034-0.070)
Female
Time to traverse	11.273 (10.182-12.364)	10.765 (9.795-11.735)	11.974 (10.392-13.556)	11.001 (9.586-12.416)
Steps	15.020 (14.308-15.732)	16.280 (15.636-16.924)**	17.180 (16.481-17.879)	15.520 (14.992-16.048)***
Errors/Step	0.046 (0.026-0.066)	0.115 (0.093-0.137)***	0.113 (0.083-0.143)	0.114 (0.085-0.143)