| Reagent | Volume | Final Concentration |
|---|---|---|
| H20 | 3.7 μl | – |
| KCl (3M) | 1 μl | 300 mM |
| HEPES (1M) | 0.2 μl | 20 mM |
| pRF4 (300ng/μl) | 1.6 μl | 50 ng/μl |
| ssODN (1μg/μl) | 2 μl | 200 ng/μl |
| sgRNA (50 μM) | 1 μl | 5 μM |
| Cas9 protein (5 μg/μl) | 0.5 μl | 0.25 μg/μl |
| Final Volume | 10 μl |
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