Boxplots of alpha diversity metrics comparing Scope, nonethanol mouthwash (Non-EtOH wash), and Saccomanno’s fixative to the OMNIgene ORAL kit for observed species (top), Faith’s PD (middle), and the Shannon index (bottom). The medians are marked by the horizontal lines in the boxes. Observations outside 1.5 times the interquartile range above the upper quartile and below the lower quartile are shown as outliers. Notches represent 95% confidence intervals for comparing medians, where nonoverlapping notches indicate evidence for differences in medians (35).