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. 2020 Jul 8;40(28):5431–5442. doi: 10.1523/JNEUROSCI.0569-20.2020

Figure 6.

Figure 6.

Excitatory inputs to ooDSGCs are more reliable after VME training. A, EPSC and IPSC of an ooDSGC in response to traveling gratings in preferred and NDs. B, Raw traces of EPSC (PD) and IPSC (ND) from two ooDSGCs in response to traveling gratings at 30% contrast. These are representative examples to illustrate more reliable VME EPSCs compared with control. C, Summary of response variability as measured by CV of peak EPSC and IPSCs at different stimulus contrasts. Traveling gratings in the PD (EPSC) and ND (IPSC) was used. Unpaired t test, p = 3.73E-02. Control, eight ooDSGCs; VME, nine ooDSGCs. D, Variability of ooDSGCs spiking responses to the onset and offset of a full field light flash at different contrasts. CV for ON (top) and OFF (bottom) responses are compared between upward VME and control groups. Two-way ANOVA with Bonferroni's multiple comparisons test, for ON (top), p = 1.19E-10; for OFF (bottom), p = 2.43E-03. Control, 73 ooDSGCs; VME, 64. E, Raw traces of ON EPSC recorded from a control and a VME ooDSGC in response to flashes at 30% contrast. These are representative examples to illustrate more reliable ON EPSCs for VME. F, Summary of response variability as measured by CV of peak EPSC and IPSCs, with ON and OFF responses separately analyzed. The stimulus was full field flashes, data for 30% and 100% contrasts are combined. Unpaired t test, p = 4.83E-02. Control, eight ooDSGCs; VME, seven ooDSGCs. G, Comparison of response reliability among different RGC types. The stimulus used is traveling gratings at 120 °/s and 100% contrast. ON-RGCs and ooDSGCs show lower CV, hence higher response reliability in the VME group as compared with the control group. OFF-RGCs: p = 0.88; control n = 77, VME n = 72; ON-RGCs: p = 3.99E-05, control n = 74, VME n = 101; ooDSGCs: p = 9.86E-07, control n = 73, VME n = 52. Unpaired t test. H, The same as G but using normalized CV. All CV values are normalized to the corresponding mean CV values of the control groups. OFF-RGCs: p = 0.80; ON-RGCs: p = 1.55E-04; ooDSGCs: p = 2.83E-03. Unpaired t test. The relative changes in CV are comparable between ON-RGC and ooDSGCs. ***p < 0.001; **p < 0.01; *p < 0.05. All error bars: SEM. Scale bars: horizontal, 250 ms; vertical, 50 pA (EPSC)/200 pA (IPSC).